According the the NCCLS Publication "Clinical Laboratory Safety; Approved Guidelines" dated Sept. 1996:

1)  "Cryostats should be decontaminated frequntly with 70% alcohol after removal off tissue debris." 

2)  "The cryostat should be defrosted and decontaminated with a tuberculocidal antiseptic at least once a week.  If a suspected tissue contains mycobacteria, immediate decontamination should be done with a tuberculocidal antiseptic."

Do you all follow both of these requirements?  I have always done #1 but not #2.

Laurie Colbert
Huntington Memorial Hospital
Pasadena, CA

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