zinc fixation/EDTA
Afternoon all!
I'm going to try zinc fixation (fixer from Pharmingen) on some mouse
limbs for 24-48hr, then decalcify in 20%EDTA. I usually decalc. after
formalin fixation but the antigens I'm after (CD4, CD19..) aren't
liking that, hence the zinc. However, I have a question about this
before I go ahead- will the EDTA somehow strip the zinc as well as
the calcium from the sections, or will it be stable after the
fixation step? My boss has raised concerns about this. He also seems
to think that "EGTA" is an excellent calcium chelator with a lower
avidity for clacium - does anyone use this instead of EDTA?
Thanks
Joey
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