paraffin for processing mice parts

From:"DiCarlo, Margaret" (by way of Histonet)


I have the opportunity to use a tissue processor to process decaled mice
forearms and hind legs. (Presently I've been manually processing which takes
much longer.)  However, I must use a combined paraplast and paraplast extra
in the tissue processor.  After 3 changes of this combined paraffin should I
put the tissue in a fourth paraffin of Tissue Prep II in a vacuum oven for
another two hours or can I just embed but embed with the Tissue Prep II ?
Will using different paraffins have any effect on cutting  the tissue and
doing future immuno's?   I would appreciate any advice.

Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital

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