Tissue processing problems

From:Kathleen Roberts (by way of Histonet)

To all-

We are a small university pathology lab that does mostly rodent and some

fish tissues, and lately they have been coming out of the processor hard

as a rock and very difficult to cut, and we have been trying to figure
out what, if anything, we are doing wrong.  We have tried changing out
the reagents more often, but it doesn't seem to have made a difference.
We doubt that it's the temperature of the paraffin, as our settings are
in agreement with the manufacturer's recommendations, and the
temperature displayed agrees with our thermometer. Could you please send

me your processing protocols for comparison?

If you like, I will send you our protocol as well.  Any and all ideas
and suggestions will be very welcome.

Thanks in advance for your help-
Kathleen Roberts
Principal Lab Technician
Neurotoxicology Laboratories
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Ernest Mario School of Pharmacy
Rutgers University
41 B Gordon Rd
Piscataway, NJ 08854
(732) 445-6914
FAX (732) 445-6905





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