Re: paraffin for processing mice parts
|From:||Jackie.O'Connor@abbott.com (by way of Histonet)|
I routinely process whole mouse limbs (decalcified) through a routine mouse
program (45 mins in graded alcohols and 55 mins x2 paraffin). I use Richard
Allen type 6 paraffin for processing and embedding (just my preference) - I
don't find the need for any additional infiltration - and my IHC and ISH
Jacqueline M. O'Connor HT(ASCP)
Global Pharmaceutical Research and Development
"DiCarlo, Margaret" (by way of Histonet)
08/13/2003 06:19 PM
Subject: paraffin for processing mice parts
I have the opportunity to use a tissue processor to process decaled mice
forearms and hind legs. (Presently I've been manually processing which takes
much longer.) However, I must use a combined paraplast and paraplast extra
in the tissue processor. After 3 changes of this combined paraffin should I
put the tissue in a fourth paraffin of Tissue Prep II in a vacuum oven for
another two hours or can I just embed but embed with the Tissue Prep II ?
Will using different paraffins have any effect on cutting the tissue and
doing future immuno's? I would appreciate any advice.
Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital
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