Snap freeze the whole brain as it is almost its own "oct" type matrix. You wouls still want to use the oct as a method for sticking the brain onto the chuck or block holder.
08/04/03 12:16 PM
Subject: flash freezing, brain, and isopentane
I have a quick question regarding flash freezing of rat brain in freezing isopentane. Does anyone have an opinion on flash-freezing by simply dropping the brain into the isopentane versus placing it in a container with OCT or similar media and then placing this into the isopentane? I have been running into problems with both micro and macro cracking of the tissue.
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