Re: controls for murine aortic immunostaining

From:Geoff McAuliffe (by way of Histonet)

Dear Dr. Francis:

    You are, according to your signature, a research fellow at a major
medical school. A very important part of your training is learning how
to find information for yourself. This means doing some real work, not
asking others to figure out your experiment and the use/function of your
experimental tools for you. This question, and the previous question you
posted, tell me that you have no idea of what you are doing or the
significance of the tools you are using. If you did, you would know the
answer to your question. If you want to learn anything from your
fellowship, start by getting a review article on immunostaining and read
it. Then apply what you have learned to your question.


sanjeev francis wrote:

> I am planning on immunostaining both  frozen sections and formalin
> fixed paraffin embedded cross sections of murine aorta and kidney to
> determine the expression of a particular protein.  I am looking for
> suggestions for antibody controls which people have had good
> experience with in these types of tissue.
> Thank you.
> Sanjeev Francis
> Research Fellow
> Weill Medical College of Cornell University
> _________________________________________________________________
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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