Re: coated slides Elmer's
|From:||firstname.lastname@example.org (by way of Histonet)|
Have you tried silane coated slides?
My experience of them is that they hold the tissue very well with next to
no background, even when you use heat mediated antigen retrieval.
> from: louise renton
> date: Thu, 07 Aug 2003 03:38:44
> to: HistoNet@pathology.swmed.edu
> subject: Re: coated slides Elmer's
> Dear all
> I have heard a lot about Elmer's Glue, which I gather is some type of
> household vinyl based adhesive. We don't get this specific brand, but
> similar ones, used specifically for woodwork or crafts are available.
> So...my question is this: How is it used? Neat, Diluted, in the
> waterbath??? PLease advise as I have some deadful whole paw sections that I
> am having difficuly "sticking" to the slide.
> BTW, I have tried chrome alum, but get horrible background so my thanks go
> to Gayle for suggesting placing them in NBF. I will try that too.
> Best regards
> Louise Renton
> Bone Research Unit
> South Africa
> Tel & fax 27 11 717 2298
> "Time flies like an arrow, fruit flies like a banana"
> ----At 08:48 a.m. 01/08/2003 -0400, you wrote:
> >I am having problems with tissue staying on my slides after drying
> >in 37C oven and then one more night in a 60C oven. The tissue is chicken
> >leg knee joints. They are formalin fixed, EDTA decaled, and paraffin
> >embedded samples. I think that coated slides may help. Does anyone have a
> >simple protocol for making coated slides to help this tissue stick?
> >Thanks in advance for the help.
> >Loralee Gehan
> >University of Rochester
> > Loralle:
> You can use a vinyl glue like Elmer#180#s, it works fine in cases
> like you are describiyng. The adhesion properties are at least
> the same that charged slides when you are treating samples
> like cartilage and bone.
> Good luck,Carlos.
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