Re: coated slides
At 08:48 a.m. 01/08/2003 -0400, you wrote:
>Hi,
>I am having problems with tissue staying on my slides after drying overnight
>in 37C oven and then one more night in a 60C oven. The tissue is chicken
>leg knee joints. They are formalin fixed, EDTA decaled, and paraffin
>embedded samples. I think that coated slides may help. Does anyone have a
>simple protocol for making coated slides to help this tissue stick?
>Thanks in advance for the help.
>
>Loralee Gehan
>University of Rochester
> Loralle:
You can use a vinyl glue like Elmerīs, it works fine in cases
like you are describiyng. The adhesion properties are at least
the same that charged slides when you are treating samples
like cartilage and bone.
Good luck,Carlos.
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