Re: Immunohistochemistry on Cell cultutres

From:"Sylvia Poulos"

Immuno on cultured cells is fairly easy if the cells attach to the vessel and aren't suspended.  If they attach, grow them in a dish instead of a flask and you can do immuno in the dish (no scraping to a slide).  If they are suspended, can you centrifuge them, pellet them, then put them on a slide?  

Sylvia P. Poulos
USDA-ARS-Animal Physiology Research Unit
Athens, GA 

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