hi all,

to everyone hello..

my question is.., where do i find vendors (usa) for pre-made hematoxylin and eosin?..cheers all,


On Tue, 5 Aug 2003, HistoNet Server wrote:

> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 03:23:18 -0500
> From: Chris van der Loos 
> Subject: RE: more info again, End PO block
> 
> Dear Becky,
> There is a new endogenous blocking solution from Dako called "Dual 
> endogenous enzyme block" (S2003). It will kill both endogenous peroxidase 
> and alkaline phosphatase activities in one 10 min incubation step prior to 
> your immunosteps. As this stuff is new my experience is only limited. So 
> far it seems very effective without damaging the tissue epitopes.
> Furthermore, as Gayle Callis already wrote, methanol + 0.3% peroxide (20 
> min, RT) is very effective to block endogenous peroxidase activity in FFPE 
> sections. Because those sections have been trough alcohols several times 
> during embedding and dewaxing, the methanol has no additional damaging 
> effect. Be aware that methanol can be very damaging to many tissue epitopes 
> when immunostaining cryostat sections!
> I have also tried to prolong the incubation time of a lesser effective 
> endogenous peroxidase blocking method as you wrote in your first mail, but 
> it didn't help.
> 
> Chris van der Loos
> Dept. of Cardiovascular Pathology
> Academical Medical Center
> Amsterdam - The Netherlands
> 
> At 11:47 AM 8/1/2003 -0400, you wrote:
>  >I'm sorry about not giving enough information.  I have had several
>  >responses asking for more details.  So, I'll try again!
>  >
>  >I am performing IHC staining.  I am using a DAKO autostainer with
>  >the LSAB detection system.  I use DAB for my chromagen.  My negative
>  >mouse and negative rabbit serum slides have quite specific staining
>  >in the granlocytic series of WBC's(No matter what pretreatment -
>  >I apply no pretreatment, Proteinase K, or use Microwave at pH 6 and
>  >10).  Microwave pretreatment is the worst, but I get the staining
>  >most of the time.  Polysegmented neutrophils seem to be the main
>  >culprit.  Most of the times my Docs can read through this, but they
>  >are requesting my experimentation to find a "cure" for the situation,
>  >so they can honestly say, "no staining was seen in the negative
>  >control". One of the suggestions I have received is to do an Avidin/Biotin
>  >Block. I will give this a try, but if there are any other ideas out
>  >there:  DON'T hold back!  THANKS AGAIN! 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 05:05:13 -0500
> From: "louise renton" 
> Subject: Re: coated slides Elmer's
> 
> Dear all
> 
> I have heard a lot about Elmer's Glue, which I gather is some type of 
> household vinyl based adhesive. We don't get this specific brand, but 
> similar ones,  used specifically for woodwork or crafts are available. 
> So...my question is this: How is it used?  Neat, Diluted, in the 
> waterbath???  PLease advise as I have some deadful whole paw sections that I 
> am having difficuly "sticking" to the slide.
> 
> BTW, I have tried chrome alum, but get horrible background so my thanks go 
> to Gayle for suggesting placing them in NBF. I will try that too.
> 
> Best regards
> 
> 
> 
> Louise Renton
> Bone Research Unit
> MRC
> Johannesburg
> South Africa
> Tel & fax +27 11 717 2298
> "Time flies like an arrow, fruit flies like a banana"
> 
> 
> 
> 
> 
> - ----At 08:48 a.m. 01/08/2003 -0400, you wrote:
> >Hi,
> >I am having problems with tissue staying on my slides after drying 
> >overnight
> >in 37C oven and then one more night in a 60C oven.  The tissue is chicken
> >leg knee joints.   They are formalin fixed, EDTA decaled, and paraffin
> >embedded samples.  I think that coated slides may help.  Does anyone have a
> >simple protocol for making coated slides to help this tissue stick?
> >Thanks in advance for the help.
> >
> >Loralee Gehan
> >University of Rochester
> 
> >   Loralle:
> You  can  use  a  vinyl glue  like  Elmer#180#s, it  works  fine  in  cases
> like you  are  describiyng. The  adhesion  properties  are  at  least
> the  same  that  charged slides  when  you are  treating  samples
> like  cartilage  and bone.
> Good luck,Carlos.
> 
> _________________________________________________________________
> Unsatisfied with being single?  Try MSN Personals 
> http://www.msn.co.za/personals/
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 10:01:46 -0500
> From: Gayle Callis 
> Subject: Caveat about Elmer's glue
> 
> Elmers glue has been used in the past, but some labs reported contamination
> (bacterial, whatever) when using it - and if I remember correctly, there
> have been publications/technical hints about this problem. That was enough
> for our lab to abandon Elmers glue in lieu of in house prepared chrome
> subbing solutions or (way back then) advent of poly l lysine or silane
> coatings.  We use Elmers glue for a short time, but disliked its white
> opacity.    
> 
> I was under the impression Elmers was a by product of the dairy industry,
> since Bordens (of Elsie the Cow fame) manufactured this glue for school
> children and household use.  Personally, I would rather purchase
> commercial(Surgipath) or make up chrome gelatin solution or some other
> slide coating solution for histology purposes and leave Elmers to the
> school children and households.   When poly l lysine coating methods
> arrived on the scene, we abandoned Elmers glue entirely or did the chrome
> gelatin subbing for the very difficult, large decalcified sheep tibia, dog
> tibia and femur section.  
> 
> If you want to reduce background and are using 275 bloom gelatin, go to 100
> bloom, larger molecule collagen often gives more H&E background staining
> than smaller molecule (100 bloom) gelatin.  
> 
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> S. 19th and Lincoln St
> Bozeman MT 59717-3610
> 
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> 
> email: gcallis@montana.edu
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 10:21:54 -0500
> From: "DiCarlo, Margaret" 
> Subject: RE: coated slides Elmer's
> 
> Louise,
> 
> Fred told me about Titebond II which is an adhesive found in any local
> hardware store. Just dilute it to make a 5% solution.  I drop a few drops on
> my slide using a disposable pipet and spread all over using kimwipe and air
> dry it right before use and I have been having great results with it.  There
> is no background staining from using it.  I hope you try this.  Good luck.
> 
> Peggy DiCarlo HT(ASCP)
> Orthopedics Bone Lab
> Buffalo General Hospital
> - -----Original Message-----
> From: louise renton [mailto:louise_renton@hotmail.com]
> Sent: Monday, August 04, 2003 06:03
> To: histonet@pathology.swmed.edu
> Subject: Re: coated slides Elmer's
> 
> 
> Dear all
> 
> I have heard a lot about Elmer's Glue, which I gather is some type of 
> household vinyl based adhesive. We don't get this specific brand, but 
> similar ones,  used specifically for woodwork or crafts are available. 
> So...my question is this: How is it used?  Neat, Diluted, in the 
> waterbath???  PLease advise as I have some deadful whole paw sections that I
> 
> am having difficuly "sticking" to the slide.
> 
> BTW, I have tried chrome alum, but get horrible background so my thanks go 
> to Gayle for suggesting placing them in NBF. I will try that too.
> 
> Best regards
> 
> 
> 
> Louise Renton
> Bone Research Unit
> MRC
> Johannesburg
> South Africa
> Tel & fax +27 11 717 2298
> "Time flies like an arrow, fruit flies like a banana"
> 
> 
> 
> 
> 
> - ----At 08:48 a.m. 01/08/2003 -0400, you wrote:
> >Hi,
> >I am having problems with tissue staying on my slides after drying 
> >overnight
> >in 37C oven and then one more night in a 60C oven.  The tissue is chicken
> >leg knee joints.   They are formalin fixed, EDTA decaled, and paraffin
> >embedded samples.  I think that coated slides may help.  Does anyone have a
> >simple protocol for making coated slides to help this tissue stick?
> >Thanks in advance for the help.
> >
> >Loralee Gehan
> >University of Rochester
> 
> >   Loralle:
> You  can  use  a  vinyl glue  like  Elmer#180#s, it  works  fine  in  cases
> like you  are  describiyng. The  adhesion  properties  are  at  least
> the  same  that  charged slides  when  you are  treating  samples
> like  cartilage  and bone.
> Good luck,Carlos.
> 
> _________________________________________________________________
> Unsatisfied with being single?  Try MSN Personals 
> http://www.msn.co.za/personals/
> 
> 
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> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 10:47:21 -0500
> From: Nick_Madary@hgsi.com
> Subject: Antibody for Anthrax Spores?
> 
> 
> I am sure this has been discussed before but are there any commercially 
> available antibodies for anthrax spores?
> 
> 
> ******************* NOTE *******************
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> contained in the following MIME Information.
> ********************************************
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> 
> 
I am sure this has been discussed before
> but are there any commercially available antibodies for anthrax spores?
> - --=_alternative 0056194085256D78_=--
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 11:14:19 -0500
> From: "Smith, Allen" 
> Subject: RE: coated slides Elmer's
> 
> Since Elmer's is made by Borden's (a dairy company), it is probably casein.
> 
> Allen A. Smith, Ph.D.
> Barry University
> School of Graduate Medical Sciences
>     Podiatric Medicine and Surgery
> Miami Shores, Florida  33161-6695 
> 
> - -----Original Message-----
> From: louise renton [mailto:louise_renton@hotmail.com]
> Sent: Monday, August 04, 2003 6:03 AM
> To: histonet@pathology.swmed.edu
> Subject: Re: coated slides Elmer's
> 
> 
> Dear all
> 
> I have heard a lot about Elmer's Glue, which I gather is some type of 
> household vinyl based adhesive. We don't get this specific brand, but 
> similar ones,  used specifically for woodwork or crafts are available. 
> So...my question is this: How is it used?  Neat, Diluted, in the 
> waterbath???  PLease advise as I have some deadful whole paw sections that I
> 
> am having difficuly "sticking" to the slide.
> 
> BTW, I have tried chrome alum, but get horrible background so my thanks go 
> to Gayle for suggesting placing them in NBF. I will try that too.
> 
> Best regards
> 
> 
> 
> Louise Renton
> Bone Research Unit
> MRC
> Johannesburg
> South Africa
> Tel & fax +27 11 717 2298
> "Time flies like an arrow, fruit flies like a banana"
> 
> 
> 
> 
> 
> - ----At 08:48 a.m. 01/08/2003 -0400, you wrote:
> >Hi,
> >I am having problems with tissue staying on my slides after drying 
> >overnight
> >in 37C oven and then one more night in a 60C oven.  The tissue is chicken
> >leg knee joints.   They are formalin fixed, EDTA decaled, and paraffin
> >embedded samples.  I think that coated slides may help.  Does anyone have a
> >simple protocol for making coated slides to help this tissue stick?
> >Thanks in advance for the help.
> >
> >Loralee Gehan
> >University of Rochester
> 
> >   Loralle:
> You  can  use  a  vinyl glue  like  Elmer#180#s, it  works  fine  in  cases
> like you  are  describiyng. The  adhesion  properties  are  at  least
> the  same  that  charged slides  when  you are  treating  samples
> like  cartilage  and bone.
> Good luck,Carlos.
> 
> _________________________________________________________________
> Unsatisfied with being single?  Try MSN Personals 
> http://www.msn.co.za/personals/
> 
> 
>            
>                        
>                 
> The information transmitted is intended only for the person or entity to which
> it is addressed and may contain confidential, and/or privileged material. No
> confidentiality or privilege is waived or lost by any errant transmission. If
> you receive this message in error, please immediately delete it and all copies
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> guaranteed to be secure or error-free as information could be intercepted,
> corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.
> Barry University - Miami Shores, FL (http://www.barry.edu) 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 11:57:39 -0500
> From: "Cathrine Dreanno" 
> Subject: RDO-gold  and ISH 
> 
> Hi, 
> 
> 
> I would like to decalcify my samples and do some in  situ hybridization.  I
> read that RDO  is not suitable  for ISH (Alers et al., 1999, 47; 703-709,
> JHC). 
> They have a new product called "RDO-gold". They say the "nucleic acid are
> altered".  Does anyone have any experience  of this product ? 
> 
> Thank you  for advices , 
> 
> 
> 
> Catherine
> 
> 
>  
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 12:20:35 -0500
> From: Margaret_McKinney@brown.edu
> Subject: flash freezing, brain, and isopentane
> 
> I have a quick question regarding flash freezing of rat brain in freezing
> isopentane. Does anyone have an opinion on flash-freezing by simply dropping
> the brain into the isopentane versus placing it in a container with OCT or
> similar media and then placing this into the isopentane? I have been running
> into problems with both micro and macro cracking of the tissue.
> 
> Thanks,
> Margaret McKinney.
> 
> Margaret McKinney
> Brown University Medical School
> 97 Waterman St.
> Providence, RI 02912
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 12:48:52 -0500
> From: Judi Ford 
> Subject: Gallyas Stain on Brain
> 
> Hi,
> 
> I've just finished doing the Gallyas stain for tangles and have found that no
> matter how gentle I am with the slides tissue falls off the slide during the
> stain.  I thought about using + slides but heard that brain tissue and plus
> slides don't work due to +/- charges working against each other.  Any
> suggestions?  Also, what could I use as a control for tangles?  I'm working
> with mouse tissue. 
> 
> Thanks for your help.
> 
> Judi Ford
> Histotechnologist
> Jackson Laboratory, ME
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 12:49:14 -0500
> From: "Dawson, Glen" 
> Subject: MIB-1 (Ki-67) VENDOR
> 
> 
> All,
> 
> I am looking for the MIB-1 (Ki-67) antibody that used to be sold by
> Immunotech (Cat. #IM0505).  Unless I'm mistaken, Immunotech is no longer
> around but this antibody is/was the best.  Any leads on who may actually
> have this particular antibody or who may have bought out immunotech would be
> much appreciated.
> 
> Thanx in Advance,
> 
> Glen A. Dawson  BS, HT & QIHC (ASCP)
> Lead IHC Technologist
> Milwaukee, WI
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 12:49:41 -0500
> From: Nick_Madary@hgsi.com
> Subject: Re: flash freezing, brain, and isopentane
> 
> 
> Snap freeze the whole brain as it is almost its own "oct" type matrix. You 
> wouls still want to use the oct as a method for sticking the brain onto 
> the chuck or block holder.
> 
> 
> 
> 
> Margaret_McKinney@brown.edu
> 08/04/03 12:16 PM
> 
>  
>         To:     histonet@pathology.swmed.edu
>         cc: 
>         Subject:        flash freezing, brain, and isopentane
> 
> 
> I have a quick question regarding flash freezing of rat brain in freezing 
> isopentane. Does anyone have an opinion on flash-freezing by simply 
> dropping the brain into the isopentane versus placing it in a container 
> with OCT or similar media and then placing this into the isopentane? I 
> have been running into problems with both micro and macro cracking of the 
> tissue.
> 
> Thanks,
> Margaret McKinney.
> 
> Margaret McKinney
> Brown University Medical School
> 97 Waterman St.
> Providence, RI 02912
> 
> 
> 
> 
> 
> 
> 
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
> 
> 
> - ------------------ MIME Information follows ------------------
> 
> This is a multipart message in MIME format.
> - --=_alternative 005E974405256D78_=
> Content-Type: text/plain; charset="us-ascii"
> 
> <<<<<< See above "Message Body" >>>>>>
> 
> - --=_alternative 005E974405256D78_=
> Content-Type: text/html; charset="us-ascii"
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> 
> 
Snap freeze the whole brain as it is almost
> its own "oct" type matrix. You wouls still want to use the oct as a
> method for sticking the brain onto the chuck or block holder.
> 

> 

> 

> 
> 
> 

> 
Margaret_McKinney@brown.edu
> 
08/04/03 12:16 PM
> 

> 
        
> 
        To:  
>      histonet@pathology.swmed.edu
> 
        cc:  
>      
> 
        Subject:  
>      flash freezing, brain, and isopentane
> 

> 

> 
I have a quick question regarding flash freezing of rat
> brain in freezing isopentane. Does anyone have an opinion on flash-freezing by
> simply dropping the brain into the isopentane versus placing it in a container
> with OCT or similar media and then placing this into the isopentane? I have
> been running into problems with both micro and macro cracking of the
> tissue.

> 

> Thanks,

> Margaret McKinney.

> 

> Margaret McKinney

> Brown University Medical School

> 97 Waterman St.

> Providence, RI 02912

> 

> 

> 
> 

> 

> - --=_alternative 005E974405256D78_=--
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 13:58:50 -0500
> From: "Jocelyn Torcolini" 
> Subject: RE: 
> 
> unsubscribe
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 14:00:12 -0500
> From: "Bryan Hewlett" 
> Subject: Re: MIB-1 (Ki-67) VENDOR
> 
> Glen,
> 
> This clone is available from DakoCytomation.
> 
> Bryan
> 
> 
> - ----- Original Message -----
> From: "Dawson, Glen" 
> To: 
> Sent: Monday, August 04, 2003 1:37 PM
> Subject: MIB-1 (Ki-67) VENDOR
> 
> 
> >
> > All,
> >
> > I am looking for the MIB-1 (Ki-67) antibody that used to be sold by
> > Immunotech (Cat. #IM0505).  Unless I'm mistaken, Immunotech is no longer
> > around but this antibody is/was the best.  Any leads on who may actually
> > have this particular antibody or who may have bought out immunotech would
> be
> > much appreciated.
> >
> > Thanx in Advance,
> >
> > Glen A. Dawson  BS, HT & QIHC (ASCP)
> > Lead IHC Technologist
> > Milwaukee, WI
> >
> >
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 14:01:01 -0500
> From: "Yaskovich, Ruth A (NIH/NIDCR)" 
> Subject: RE: Gallyas Stain on Brain
> 
> I cut mouse brain all the time on plus slides from statlabs with no problem
> with tissue falling off.
> Ruth Yaskovich
> 
> 
> - -----Original Message-----
> From: Judi Ford [mailto:jlf@jax.org] 
> Sent: Monday, August 04, 2003 1:34 PM
> To: histonet@pathology.swmed.edu
> Subject: Gallyas Stain on Brain
> 
> Hi,
> 
> I've just finished doing the Gallyas stain for tangles and have found that
> no matter how gentle I am with the slides tissue falls off the slide during
> the stain.  I thought about using + slides but heard that brain tissue and
> plus slides don't work due to +/- charges working against each other.  Any
> suggestions?  Also, what could I use as a control for tangles?  I'm working
> with mouse tissue. 
> 
> Thanks for your help.
> 
> Judi Ford
> Histotechnologist
> Jackson Laboratory, ME
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Aug 2003 14:01:43 -0500
> From: "Wright, Barbara (DNAX)" 
> Subject: RE: MIB-1 (Ki-67) VENDOR
> 
> Glen,
> 
> I belive you can get Immunotech products through Becton Dickinson.
> DAKO's Ki-67 MIB-1 antibody works great too.
> 
> Hope that helps
> Barb Wright
> 
> - -----Original Message-----
> From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
> Sent: Monday, August 04, 2003 10:38 AM
> To: histonet@pathology.swmed.edu
> Subject: MIB-1 (Ki-67) VENDOR
> 
> 
> 
> All,
> 
> I am looking for the MIB-1 (Ki-67) antibody that used to be sold by
> Immunotech (Cat. #IM0505).  Unless I'm mistaken, Immunotech is no longer
> around but this antibody is/was the best.  Any leads on who may actually
> have this particular antibody or who may have bought out immunotech would be
> much appreciated.
> 
> Thanx in Advance,
> 
> Glen A. Dawson  BS, HT & QIHC (ASCP)
> Lead IHC Technologist
> Milwaukee, WI
> 
> 
> *********************************************************************
> This message and any attachments are solely for the intended recipient. If you
> are not the intended recipient, disclosure, copying, use or distribution of
> the information included in this message is prohibited -- Please immediately
> and permanently delete.
> 
> 
> 
> Here are the messages received yesterday!
> 
>