RE: paraffin for processing mice parts

From:"DiCarlo, Margaret" (by way of Histonet)

>  -----Original Message-----
> From: 	DiCarlo, Margaret
> Sent:	Wednesday, August 13, 2003 13:17
> To:	''
> Subject:	 paraffin for processing mice parts
> Histonetters,
> I have the opportunity to use a tissue processor to process decaled mice
> forearms and hind legs. (Presently I've been manually processing which
> takes much longer.)  However, I must use a combined paraplast and
> paraplast extra in the tissue processor.  After 3 changes of this combined
> paraffin should I put the tissue in a fourth paraffin of Tissue Prep II in
> a vacuum oven for another two hours or can I just embed but embed with the
> Tissue Prep II ?  Will using different paraffins have any effect on
> cutting  the tissue and doing future immuno's?   I would appreciate any
> advice.
> Peggy DiCarlo HT (ASCP)
> Orthopedics Bone Lab
> Buffalo General Hospital

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