At 07:37 a.m. 08/08/2003 +0000, you wrote:
>Dear Fred,
>
>Sadly, titebond is not available in South Africa. I have made extensive
>enquiries, but with no luck. So unless the formula is available (nothing
>on the Franklin website) I can't even guess for a substitute.
>
>Thanks anyway
>
>Louise:

What you need is not a  specific trade mark. You  only  have  to  purchase
a vinyl glue like those for scholar use and thatīs all.
In my country (Uruguay),we find different brands like Elmerīs,
Titebond, Cascola,etc. All of them work the  same  way. Make a
fresh dilution of the glue in water,removing to achieve homogeneity
being the solution of white colour.Discard the solution after use,and make
a fresh one when you need to.
My regards,Carlos.


>Louise Renton
>Bone Research Unit
>MRC
>Johannesburg
>South Africa
>Tel & fax +27 11 717 2298
>"Time flies like an arrow, fruit flies like a banana"
>
>
>Dear Fred,
>
>sa
>
>
>----
>
>Hi Louise.  I use a product called titebond.  It is a water
>resistant wood glue.  If you are unable to find that brand I'm sure
>that other wood glues would work.  I make an aqueous solution of 1%-5%
>(it is thick and difficult to measure an exact amount) and add a grain
>of thymol for preservative.  I spread a small drop on a slide and
>allow it to dry before mounting the section.  It does a great job of
>adhereing dense bone.  Unlike Elmers glue or gelatin, there is no
>background staining.
>
>Fred Underwood
>Montgomery County Coroner
>Dayton, Ohio
>Subject: Re: coated slides Elmer's
>
>
>
>         Dear all
>
>         I have heard a lot about Elmer's Glue, which I gather is some
>type of
>household vinyl based adhesive. We don't get this specific brand, but
>similar ones,  used specifically for woodwork or crafts are
>available.
>So...my question is this: How is it used?  Neat, Diluted, in the
>waterbath???  PLease advise as I have some deadful whole paw sections
>that I
>am having difficuly "sticking" to the slide.
>
>         BTW, I have tried chrome alum, but get horrible background so my
>thanks go
>to Gayle for suggesting placing them in NBF. I will try that too.
>
>         Best regards
>
>
>
>         Louise Renton
>Bone Research Unit
>MRC
>Johannesburg
>South Africa
>Tel & fax +27 11 717 2298
>"Time flies like an arrow, fruit flies like a banana"
>
>
>
>
>
>             At 08:48 a.m. 01/08/2003  0400, you wrote:
> >Hi,
> >I am having problems with tissue staying on my slides after drying
> >overnight
> >in 37C oven and then one more night in a 60C oven.  The tissue is
>chicken
> >leg knee joints.   They are formalin fixed, EDTA decaled, and
>paraffin
> >embedded samples.  I think that coated slides may help.  Does
>anyone have a
> >simple protocol for making coated slides to help this tissue stick?
> >Thanks in advance for the help.
> >
> >Loralee Gehan
> >University of Rochester
>
>         >   Loralle:
>You  can  use  a  vinyl glue  like  Elmer#180#s, it
>works  fine  in  cases
>like you  are  describiyng. The  adhesion  properties  are
>at  least
>the  same  that  charged slides  when  you are  treating
>samples
>like  cartilage  and bone.
>Good luck,Carlos.
>
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