Since Elmer's is made by Borden's (a dairy company), it is probably casein.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695 

-----Original Message-----
From: louise renton [mailto:louise_renton@hotmail.com]
Sent: Monday, August 04, 2003 6:03 AM
To: histonet@pathology.swmed.edu
Subject: Re: coated slides Elmer's


Dear all

I have heard a lot about Elmer's Glue, which I gather is some type of 
household vinyl based adhesive. We don't get this specific brand, but 
similar ones,  used specifically for woodwork or crafts are available. 
So...my question is this: How is it used?  Neat, Diluted, in the 
waterbath???  PLease advise as I have some deadful whole paw sections that I

am having difficuly "sticking" to the slide.

BTW, I have tried chrome alum, but get horrible background so my thanks go 
to Gayle for suggesting placing them in NBF. I will try that too.

Best regards



Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





----At 08:48 a.m. 01/08/2003 -0400, you wrote:
>Hi,
>I am having problems with tissue staying on my slides after drying 
>overnight
>in 37C oven and then one more night in a 60C oven.  The tissue is chicken
>leg knee joints.   They are formalin fixed, EDTA decaled, and paraffin
>embedded samples.  I think that coated slides may help.  Does anyone have a
>simple protocol for making coated slides to help this tissue stick?
>Thanks in advance for the help.
>
>Loralee Gehan
>University of Rochester

>   Loralle:
You  can  use  a  vinyl glue  like  Elmer#180#s, it  works  fine  in  cases
like you  are  describiyng. The  adhesion  properties  are  at  least
the  same  that  charged slides  when  you are  treating  samples
like  cartilage  and bone.
Good luck,Carlos.

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