RE: coated slides Elmer's
Since Elmer's is made by Borden's (a dairy company), it is probably casein.
Allen A. Smith, Ph.D.
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695
From: louise renton [mailto:firstname.lastname@example.org]
Sent: Monday, August 04, 2003 6:03 AM
Subject: Re: coated slides Elmer's
I have heard a lot about Elmer's Glue, which I gather is some type of
household vinyl based adhesive. We don't get this specific brand, but
similar ones, used specifically for woodwork or crafts are available.
So...my question is this: How is it used? Neat, Diluted, in the
waterbath??? PLease advise as I have some deadful whole paw sections that I
am having difficuly "sticking" to the slide.
BTW, I have tried chrome alum, but get horrible background so my thanks go
to Gayle for suggesting placing them in NBF. I will try that too.
Bone Research Unit
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"
----At 08:48 a.m. 01/08/2003 -0400, you wrote:
>I am having problems with tissue staying on my slides after drying
>in 37C oven and then one more night in a 60C oven. The tissue is chicken
>leg knee joints. They are formalin fixed, EDTA decaled, and paraffin
>embedded samples. I think that coated slides may help. Does anyone have a
>simple protocol for making coated slides to help this tissue stick?
>Thanks in advance for the help.
>University of Rochester
You can use a vinyl glue like Elmer#180#s, it works fine in cases
like you are describiyng. The adhesion properties are at least
the same that charged slides when you are treating samples
like cartilage and bone.
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