Louise,

Fred told me about Titebond II which is an adhesive found in any local
hardware store. Just dilute it to make a 5% solution.  I drop a few drops on
my slide using a disposable pipet and spread all over using kimwipe and air
dry it right before use and I have been having great results with it.  There
is no background staining from using it.  I hope you try this.  Good luck.

Peggy DiCarlo HT(ASCP)
Orthopedics Bone Lab
Buffalo General Hospital
-----Original Message-----
From: louise renton [mailto:louise_renton@hotmail.com]
Sent: Monday, August 04, 2003 06:03
To: histonet@pathology.swmed.edu
Subject: Re: coated slides Elmer's


Dear all

I have heard a lot about Elmer's Glue, which I gather is some type of 
household vinyl based adhesive. We don't get this specific brand, but 
similar ones,  used specifically for woodwork or crafts are available. 
So...my question is this: How is it used?  Neat, Diluted, in the 
waterbath???  PLease advise as I have some deadful whole paw sections that I

am having difficuly "sticking" to the slide.

BTW, I have tried chrome alum, but get horrible background so my thanks go 
to Gayle for suggesting placing them in NBF. I will try that too.

Best regards



Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





----At 08:48 a.m. 01/08/2003 -0400, you wrote:
>Hi,
>I am having problems with tissue staying on my slides after drying 
>overnight
>in 37C oven and then one more night in a 60C oven.  The tissue is chicken
>leg knee joints.   They are formalin fixed, EDTA decaled, and paraffin
>embedded samples.  I think that coated slides may help.  Does anyone have a
>simple protocol for making coated slides to help this tissue stick?
>Thanks in advance for the help.
>
>Loralee Gehan
>University of Rochester

>   Loralle:
You  can  use  a  vinyl glue  like  Elmer#180#s, it  works  fine  in  cases
like you  are  describiyng. The  adhesion  properties  are  at  least
the  same  that  charged slides  when  you are  treating  samples
like  cartilage  and bone.
Good luck,Carlos.

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