RE: coated slides Elmer's
Fred told me about Titebond II which is an adhesive found in any local
hardware store. Just dilute it to make a 5% solution. I drop a few drops on
my slide using a disposable pipet and spread all over using kimwipe and air
dry it right before use and I have been having great results with it. There
is no background staining from using it. I hope you try this. Good luck.
Peggy DiCarlo HT(ASCP)
Orthopedics Bone Lab
Buffalo General Hospital
From: louise renton [mailto:firstname.lastname@example.org]
Sent: Monday, August 04, 2003 06:03
Subject: Re: coated slides Elmer's
I have heard a lot about Elmer's Glue, which I gather is some type of
household vinyl based adhesive. We don't get this specific brand, but
similar ones, used specifically for woodwork or crafts are available.
So...my question is this: How is it used? Neat, Diluted, in the
waterbath??? PLease advise as I have some deadful whole paw sections that I
am having difficuly "sticking" to the slide.
BTW, I have tried chrome alum, but get horrible background so my thanks go
to Gayle for suggesting placing them in NBF. I will try that too.
Bone Research Unit
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"
----At 08:48 a.m. 01/08/2003 -0400, you wrote:
>I am having problems with tissue staying on my slides after drying
>in 37C oven and then one more night in a 60C oven. The tissue is chicken
>leg knee joints. They are formalin fixed, EDTA decaled, and paraffin
>embedded samples. I think that coated slides may help. Does anyone have a
>simple protocol for making coated slides to help this tissue stick?
>Thanks in advance for the help.
>University of Rochester
You can use a vinyl glue like Elmer#180#s, it works fine in cases
like you are describiyng. The adhesion properties are at least
the same that charged slides when you are treating samples
like cartilage and bone.
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