VWR/Merck/BDH sell a Glycerol based product called Molyfex which does this
job very well.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England


-----Original Message-----
From: Favara, Cynthia (NIH/NIAID) [mailto:cfavara@niaid.nih.gov]
Sent: 01 August 2003 16:05
To: 'Laurie Colbert'; histonet@pathology.swmed.edu
Subject: RE: Hydrating tissue


I work with lots of crunchy rodent tissue and sometimes will soak in 10%
sterile glycerol. Might want to give that a try. I only use sterile when I
prepare the glycerol so it keeps on the shelf without growing things!!!

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: Laurie Colbert [mailto:laurie.colbert@huntingtonhospital.com]
Sent: Thursday, July 31, 2003 1:10 PM
To: histonet@pathology.swmed.edu
Subject: Hydrating tissue


We had a prostate needle biopsy today (again!) that was very crunchy and
hard to cut.  Our other biopsies were fine.  One of our pathologists heard
something about re-hydrating tissue with glycerin.  Does anyone know what
this technique might be?

Laurie Colbert
Huntington Memorial Hospital
Pasadena, CA
(626) 397-5784