Same here cut mouse brains and put on superfrost from fisher

C Favara

-----Original Message-----
From: Yaskovich, Ruth A (NIH/NIDCR)
[mailto:ryaskovich@dir.nidcr.nih.gov]
Sent: Monday, August 04, 2003 12:37 PM
To: 'Judi Ford'; histonet@pathology.swmed.edu
Subject: RE: Gallyas Stain on Brain


I cut mouse brain all the time on plus slides from statlabs with no problem
with tissue falling off.
Ruth Yaskovich


-----Original Message-----
From: Judi Ford [mailto:jlf@jax.org] 
Sent: Monday, August 04, 2003 1:34 PM
To: histonet@pathology.swmed.edu
Subject: Gallyas Stain on Brain

Hi,

I've just finished doing the Gallyas stain for tangles and have found that
no matter how gentle I am with the slides tissue falls off the slide during
the stain.  I thought about using + slides but heard that brain tissue and
plus slides don't work due to +/- charges working against each other.  Any
suggestions?  Also, what could I use as a control for tangles?  I'm working
with mouse tissue. 

Thanks for your help.

Judi Ford
Histotechnologist
Jackson Laboratory, ME