RE: Gallyas Stain on Brain

From:"Yaskovich, Ruth A (NIH/NIDCR)"

I cut mouse brain all the time on plus slides from statlabs with no problem
with tissue falling off.
Ruth Yaskovich

-----Original Message-----
From: Judi Ford [] 
Sent: Monday, August 04, 2003 1:34 PM
Subject: Gallyas Stain on Brain


I've just finished doing the Gallyas stain for tangles and have found that
no matter how gentle I am with the slides tissue falls off the slide during
the stain.  I thought about using + slides but heard that brain tissue and
plus slides don't work due to +/- charges working against each other.  Any
suggestions?  Also, what could I use as a control for tangles?  I'm working
with mouse tissue. 

Thanks for your help.

Judi Ford
Jackson Laboratory, ME

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