From: | Terri Thinnes |
Hi Lin, I used the Dako antibody to Ki-67 with this protocol on FFPE mouse tissue. Best of luck. HIER citrate 0.01M pH 6.0 microwave 5 min. 10% NGS 15 min Ki-67 1:20 in PBS 30 min RT GAR, bio, 1:50 Zymed 62-9540 ABC, Vector elite kit, 30 min RT NovaRed chromogen 2 min RT