Inadequately fixed bone

From:"Chan Wai Kam"

Hi Histonetters,

I've not encountered this problem before so need some advice.  I am
processing some guinea pig knee joints.  I placed them in NBF for a week
and they are now decalcifying in 30% formic acid.  From their appearance
(still slightly reddish and soft), I suspect they are not properly
fixed. Fixed tissues usually appear compact and yellowish. Is it
possible for me to reverse the procedure back to fixation again or are
my specimens beyond salvaging?  Would appreciate your advice.

Julee C
Orthopaedic Surgery
National University of Singapore
E-mail :  

<< Previous Message | Next Message >>