Immunohistochemistry on Cell cultutres

From:LD Ridley (by way of Histonet)

Hi everyone,
             I know this may ba a broad question, but does anyone have a
protocol that they are willing to share for carrying out
immunohistochemistry on cell cultures.
In our lab we have lots of experience of immuno histochemistry on tissue
sections on slides aswell as another department who are experienced in cell
culture, but I would just like to know the stepping stone in between. In
particular how you take your cells from the culture medium in the flasks
and transfer them to the slide follwing fixation etc ready for immuno.
Many thanks in advance for your help.
Lee Ridley
Research Assistant

Birmingham Women's Hospital
Reproductive & Child Health
Academic Unit 3RD Floor
United Kingdom
B15 2TG

Tel: +44 (0)121 6074754

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