|From:||"Mcleod, H, Heather" |
Dear All Has anybody used the Biomeda polyclonal antibody (V2056) to Muc3? While the spec sheet recommends 1:100, I have diluted up to 1:2000 and still get staining in nornal stomach which is NOT supposed to happen, no antigen retrieval either. Any help will be greatly appreciated. Also, Calponin (basic) I am not having much luck. What do other IHC labs do?