Re: Prussian Blue rxn in bone, no postive rxn.
What type of fixative was used?
----- Original Message -----
Sent: Thursday, August 22, 2002 4:15
Subject: Prussian Blue rxn in bone, no
currently working on my HTL designation. One of the requested specimens for my
practical is bone with the cortex and marrow. The bone is to be stained for
iron with a Prussian blue reaction.
I have tried many different bone
specimens using the Perls method with no positive reaction, but the control
(liver) is positive. One specimen the pathologist believed he saw hemosiderin
in the H&E, but with the Perls again being negative. :(
All of the
glass ware was chemically clean. I used 10% Potassium Ferrocyanide and 10% HCL
all fresh. I initially tried 5 minutes in Potassium Ferrocyanide and then 10
minutes with no results. I have used slow decal (formical) and overnight
decal. Is the acid content in these products causing the loss of iron?
of the specimens are animal (dog and min-pigs) . Would human bone exhibit more
I would appreciate any suggestions to changes in processing or
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