Re: EDTA antigen retrieval

"Klassen, Shannon SDH" 
2002-08-29 09:30

        To:     'Histonet' 
        Subject:        EDTA antigen retrieval

Can some please send me a recipe for EDTA antigen retrieval solution. 
Shannon Klassen
IHC Tech
Saskatoon Health District
Saskatoon, Canada


You can use 1mM EDTA (pH 8.0) solution for antigen retreival.   Add 0.37g 
EDTA (Sigma cat#E-5134) to 1 liter of distilled water. Adjust to pH 8.0 
with  1.0  M Sodium Hydroxide. Hope this helps.  Tom.

Tom Wells BSc, ART
Supervisor, Immunohistochemistry Laboratory
Lions Gate Hospital
North Vancouver, BC

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