I don't see the need for agar at all. When I first worked at the Queens
Medical Center in Honolulu, the techs were using agar for processing
fragmented biopsies a long standing practice. The problem was if the agar
didn't cure properly for WHATEVER reason, it would be a bear to cut. Most of
the time, the fragments were not on the same plane in the agar, anyway. A
good wrap job with lens or other appropriate paper and good embedding technic
eliminated the long standing belief in agar.
Need some aid. Does anyone use agar to support small tissues before embedding?
If so, can you share a recipe? I have little experience with formulas, how to
The process has been in place for some time here but too often, I hear
complaints like "agar is difficult to cut today." 3 gms of agar in 100 cc NBF
heated. Any ideas?
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