Re: Agar
We use a 4.0% agar solution in distilled water (refrig. storage,
heating before use) but mostly for EM specimens ( such as nasal
cilia swabs, urine samples). Have used this 4.0% solution in
regular and microwave processing with excellant results. Never
have had a problem with sectioning.
Jean Mitchell, BS, HT
University of Wisconsin Hospital and Clinics
Department of Neurology
Madison, WI
7F00,0000,0000> Need some aid. Does anyone use agar to support small tissues before
> embedding? If so, can you share a recipe? I have little experience
> with formulas, how to make, etc. The process has been in place for
> some time here but too often, I hear complaints like "agar is
> difficult to cut today." 3 gms of agar in 100 cc NBF heated. Any
> ideas? Thanks
>
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