RE: does TB grow out of formalin fixed tissue?

From:"Marshall Terry Dr, Consultant Histopathologist"

Anecdotal only, but when I was a little boy pathologist in Sheffield, England, the neuropathologists grew M. tuberculosis from a brain that had been in formalin at least the normal (for neuropathologists) six weeks.

Terry L Marshall B.A.(Law), M.B.Ch.B., F.R.C.Path
Consultant Histopathologist
Rotherham General Hospital, Yorkshire 


-----Original Message-----
From: Vinnie Della Speranza []
Sent: 28 August 2002 16:15
Subject: does TB grow out of formalin fixed tissue?

one of our pathologists indicated to me that she is certain that TB has been grown from formalin fixed tissue. She also believes this is true for HIV. This information was offered to contradict a statement I made to a resident that formalin fixation neutralizes blood borne pathogens.
I'd appreciate hearing from anyone who can speak to this issue ( efficacy of formalin  to neutralize infectious organisms, especifically on the infectious potential of TB, HIV and Hep C)  with some authority.
Of course there is always the possibility that tissues had not been adequately exposed to formalin which permitted some organisms to escape its effects and then grow in culture, as for example, when large organs are dropped in formalin without breadloafing.
appreciate your thoughts
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

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