RE: Immunofluorescence or histochemistry methods for mitochondria l stain

From:

Tony Henwood

Alessandra,

Firstly, there is no such thing as "4% paraformaldehyde fixed/paraffin embedded sections ". It is 4% formaldehyde, paraformaldehyde is a powder.

To visualise mitochondria you can use the NADH or SDH demonstration. (The NADH seems to stain more strongly), or you can use Gomori's Trichrome on air-dried frozen sections. I can send you these methods via email if you like. They are relatively easy to do.

Dr Richard Cartun [Rcartun@harthosp.org] has good success using an anti-mitochondrial antibody on paraffin sections (should be in Histonet archives).

Hope this helps

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: Alessandra Livraghi [mailto:alessandra_livraghi@med.unc.edu]
Sent: Wednesday, 28 August 2002 7:03
To: HistoNet Server
Subject: Immunofluorescence or histochemistry methods for mitochondrial stain

Hallo everybody,

I have some frozen sections and 4% paraformaldehyde fixed/paraffin embedded sections of respiratory epithelium I would like to stain for mitochondria. I'm trying some immunofluorescence methods, but looking for suggestions in HistoNet I found out that there are also some histochemistry methods to visualize mitochondria in tissues (Cain's, Novelli...).

Since I'm not an experienced histologist, can anybody give me some advices/references (where maybe I could find a detailed protocol) about these methods?

Thank you very much in advance!

Alessandra

Dr. Alessandra Livraghi, PhD
UNC School of Medicine
CF/Pulmonary Research and Treatment Center
CB#7248 7013 Thurston-Bowles Bldg.
Chapel Hill NC 27599
tel 919-966-7045
fax 919-966-7524





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