RE: How does acid alcohol / picric acid "set" ink on tissue?
|From:||firstname.lastname@example.org (Emmanuel Maicas)|
It basically fixes the surface layer of tissue, thus coagulating the ink
with the serum proteins. Formalin does the same thing but more slowly. I
never use these solutions because it introduces a lot of acid in the final
fixative (phosphate-buffered formalin), which does not have enough buffering
capacity to neutralize it (measure the pH the next morning!). I suspect this
acid is a source of variation in IHC staining for estrogen receptors in
breast cancer. I simply ink and blot dry with several changes of gauze or
paper towel before cutting the tissue - works well! It also eliminates the
problem of storing picric acid powder.
Emmanuel Maicas, Pathologist in Moncton, Canada
From: Alex Knisely [mailto:email@example.com]
Sent: Friday, August 30, 2002 4:26 AM
To: HistoNet Server
Subject: How does acid alcohol / picric acid "set" ink on tissue?
Mechanism question, with some background in the text below:
Through what steps does immersion in Bouin's fluid (with picric acid) or
acid alcohol "set" India ink, or other cutting-in station coloured inks,
Somehow I expect that Dr Monson will have this information at his fingers'
Best thanks for any replies
>X-MimeOLE: Produced By Microsoft Exchange V6.0.5762.3
>Subject: RE: Thanks --
>Date: Thu, 29 Aug 2002 14:23:07 -0400
>Thread-Topic: Thanks --
>From: "Smith, Allen"
>To: "Alex Knisely"
>X-MailScanner: Found to be clean
>The principal active ingredient in Bouin's fluid is picric acid, which is
>mostly ionized above
>pH 2. In some cases picric acid does act as a mordant. I don't think it
>does in this case. "Mordanting" implies a particular mechanism; I use the
>word only where there is evidence for such a mechanism.
>Allen A. Smith, Ph.D.
>School of Graduate Medical Sciences
> Podiatric Medicine and Surgery
>Miami Shores, Florida 33161-6695
>From: Alex Knisely [mailto:firstname.lastname@example.org]
>Sent: Thursday, August 29, 2002 2:25 AM
>To: Smith, Allen
>Subject: Thanks --
>-- is it thus wrong to write (or as I do to dictate during gross
>descriptions) "The resection margins are inked black and the ink is
>mordanted in Bouin's fluid / acid alcohol"?
>At 12:23 28/08/02 -0400, you wrote:
>> A mordant is an ion, almost always metallic, that binds to something
>>the tissue and to the dye. a dye molecule that binds to the mordant or
>>directly to a part of the tissue. Allen A. Smith, Ph.D.
>>School of Graduate Medical Sciences
>> Podiatric Medicine and Surgery
>> 33161-6695 -----Original Message-----
>>From: kevin williams [mailto:email@example.com]
>>Sent: Wednesday, August 28, 2002 11:23 AM
>>To: HistoNet Server
>>Subject: The difference between a Mordant and an Auxocrome
>> are they one in the same. Thanks for all your help Kev.
>> Get more from the Web. FREE MSN Explorer download :
>Alex Knisely, MD
>Institute of Liver Studies
>King's College Hospital
>London SE5 9RS UK
>+44 (0)20 - 7346 - 3125 telefax
>+44 (0)20 - 7346 - 4627 office
Alex Knisely, MD
Institute of Liver Studies
King's College Hospital
London SE5 9RS UK
+44 (0)20 - 7346 - 3125 telefax
+44 (0)20 - 7346 - 4627 office
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