RE: Embedding

From:"Marshall Terry Dr, Consultant Histopathologist"

Hi Agustin,

We do it simply as follows. The mold on a hot plate is part  or fully filled with wax, then the tube is positioned vertically with forceps, and the mold raised a little from the hotplate, shortly after which the base goes turbid as the wax sets. This is then quickly passed onto the cooling area. This may be done as a one or 2 stage operation depending on the length and/or floppiness of the tube. 
There does not appear to be much difficulty in this operation.

For Andrea:   <<<<<<

"...metricel membrane they get from VWR..."
What on earth is this? :-)

Terry L Marshall B.A.(Law), M.B.Ch.B., F.R.C.Path
Consultant Histopathologist
Rotherham General Hospital, Yorkshire

-----Original Message-----
From: Agustín Venzano []
Sent: 29 August 2002 15:48
To: Histonet Server
Subject: Embedding

Good morning to all netters! I'm writing to you from Buenos Aires,
Argentina, at 11.30 a.m. on August 29. Regarding the "embedding matter"
recently discussed in our list here goes our opinion and experience.
As we routinely process tissues from quite different animal species, some of
them being tiny e.g. rodents, birds; and other rather huge e.g. catte,
horses, we decided to adopt the following embedding method: We first orient
all samples attaching them to the bottom of the corresponding molds, and
finally we pour the melted paraffin. Whoever has worked with rodents' or
birds' tubular viscera e.g. intestines, has seen how difficult it is to
manipulate and correctly orient these pieces. Our trick consists of quickly
submerging one tip of the piece into melted paraffin and stick it to the
empty mold in a vertical position. Now it will be ready for embedding. We
've never attempted to do it the other way round. What are your thoughts and
how do the Histonetters belonging to the "other embedding school" to achieve
a vertical orientation of minute tubular viscera? I'd be pleased to read Dr.
Marshall's opinion on this subject. Hi Terry how are you?

Sincerely yours

Agustin J. Venzano
DVM, INTA, Argentina


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