You may want to try the COX-2 ab's (mono or polyclonal) from Oxford
Biomedical, AR in citrate buffer and O/N incubation at 4C.
Brett

Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Neuroscience
WP26A-3000
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075
e-mail. brett_connolly@merck.com


-----Original Message-----
From: Greg Dobbin [mailto:dobbin@Upei.CA]
Sent: Monday, August 19, 2002 12:47 PM
To: Allie, N, Nafiesa,
Cc: histonet@pathology.swmed.edu
Subject: Re: Cox-2 antibody



Nafiesa,
I am still struggling with my COX-2 staining as well; but you might try 
incubating your primary antibody overnight at 4 C.  Good luck.


Date sent:      	Mon, 19 Aug 2002 08:49:27 +0200
From:           	"Allie, N, Nafiesa," 
Subject:        	Cox-2 antibody
To:             	histonet@pathology.swmed.edu

> Hi Everyone
> I'm hoping that somebody will have some advise about the Cox-2 
> antibody from BD Transduction Laboratories, Cat#  610203.
> I've tried antigen retrieval with Citrate Buffer, diluting the 
> antibody at 1:100, incubating for 30min, and using Envision(Dako), 
> with no success. If anyone can give some advise, it will be very 
> appreciated.
> Thank you..
> Nafiesa
>
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> Nafiesa Allie
> Dept. Anat.Path.
> Medical School
> Univ.of Cape Town
> Anzio Rd.
> Observatory
> 7925
> Rep. of South Africa
>
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> 


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
"A farmer is a person outstanding in their field."



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