Prussian Blue rxn in bone, no postive rxn.
I am currently working on my HTL designation. One of the requested specimens
for my practical is bone with the cortex and marrow. The bone is to be
stained for iron with a Prussian blue reaction.
I have tried many different bone specimens using the Perls method with no
positive reaction, but the control (liver) is positive. One specimen the
pathologist believed he saw hemosiderin in the H&E, but with the Perls again
being negative. :(
All of the glass ware was chemically clean. I used 10% Potassium Ferrocyanide
and 10% HCL all fresh. I initially tried 5 minutes in Potassium Ferrocyanide
and then 10 minutes with no results. I have used slow decal (formical) and
overnight decal. Is the acid content in these products causing the loss of
All of the specimens are animal (dog and min-pigs) . Would human bone exhibit
I would appreciate any suggestions to changes in processing or staining
<< Previous Message | Next Message >>