Embedding and adhesion problems.
Lorraine,
I agree with all that Ross Stapf has
mentioned. We embedded from wax for many years and then changed to no wax
with little problems. The secret is to have the block storage area a
little hotter than when filled with wax and to keep the lid closed as often as
possible.
Sections floating with anystain are usually a good
indication of not enough TIME in the oven. We also use an
oven with a fan to move the air and dry routine slides for 15min at 60C,
immunoperoxidase for 30mins minimum at 60C. We have been using Superfrost
Plus slides for the last 15 years. To help alleviate this problem.
All the best
Bill Sinai
Laboratory Manager
Tissue
Pathology
ICPMR
P.O. Box 533
Wentworthville NSW
2145
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