Alkaline Phosphatase for meningiomas-HELP!

From:"R.Z. Meitus"

Hello All,

I have been asked by one of our neuropathologists to
do an alk phos on frozen sections of meningiomas.  I
have tried 2 methods so far with no results.
One method was
http://www.nottingham.ac.uk/pathology/default.html
(not the APAAP method);
and the other was out of the Freida Carson text.
I've searched the histonet archives and found that
someone suggested using an alk phos kit (e.g. Vector).
The vector catalog says that these kits are not
recommended for staining neuro stuff.
Any help will be greatly appreciated, esp. someone
with a protocol.

Thank you in advance,
Rebecca Z. Meitus, HTL


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