1) Karnovsky Fix 2) Michel's \"Fixative\"
I will be receiving tissue from another lab, some of which will be fixed in
Karnovsky. (My EM people want paraffin embedded tissue and EM tissue from the
same sample), so:
1a) Because of the gluteraldehye content of the fix will I need to re-work my
Abs dilutions ?
1b) All the Abs I will be using require enzyme or HIER for FFPE tissue, so will
I need to extend times?
2a) I think I read that tissue for EM cannot be transported in Michel's. Is
2b) I have never used Michel's, so suggested times and solutions for rinsing
before fixing/freezing would be appreciated.
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