storage of frozen tissue
Instrumedics' Protective Oil ,Cat#PO, was developed for coating the frozen
block to protect it from dehydration in long term storage at -80C. The oil
remains a liquid until -10C so that it can be brushed on the frozen block
without melting the tissue. When it is brushed on to the tissue which is
below -10C it freezes and offers the protection from dehydration which
takes place over time in a freezer.
When the block is to be sectioned the solidified oil coat is trimmed away
when facing off the block to expose the tissue.
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