ploidy and % S phase

From:Martha Ward

I was wondering what everyone is doing for their breast cancer specimens in regards to immunos and/or flow cytometry?  We currently do ER, PR, Her-2 (Dako), Ki-67, as well as ploidy and %S phase by flow cytometry.  We have not changed our menu in some time and I wanted to get a feel for what other institutions are doing.  My impression from talking with some oncologists is that they do not need the ploidy/% S phase but we keep getting requests.  I wonder if this is one of those "we've always done it" situations.  I would appreciate any input while we evaluate the situation.  Thanks in advance for your input.
 
Martha Ward, MT (ASCP) QIHC
Wake Forest University Baptist Medical Center
Molecular Diagnostics Lab 




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