microglial protocol?

From:Jan Bauer

Dear Janet,

As far as I am aware there are no specific immunostainings for "resting microglia", at least not working on paraffin. In the rat you can stain microglia on cryostate sections with OX-42 (complement receptor 3). In pathological situations the microglia start upregulation a number of antigens with which they than can be recognised. These include for rat: ED1 (lysosomal marker), OX-6 (MHC class II), both working on paraffin after heat-induced antigen retrieval. Another way which you can use are histochemical stainings with lectins. In the rat this would be Griffonia simlicifolia isolectin-B4 (can be bought by Sigma). In the cat I do not know, possibly the same. The lectin staining also works on paraffin and stains all microglial cells (also the resting).

best wishes

Jan Bauer, Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse 4
A-1090 Vienna

tel:    +43-1-4277 62813
fax:    +43-1-4277 9628

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