embedding non-tissue material

From:Anthony Choo

Hello,

I am trying to embed some soft tissue under residual compression. (i.e. I
want to be able to look at compressed structures under the microscope.) I've
used nylon screws to apply the compression and embedded in JB4 plus.  The
specimen does not cut well on a glass microtome (I presume because the
plastic cannot be inflitrated giving me a compliance mismatch).  Any
suggestions for alterate materials?

(Note: I've considered using a device made of, say wood, however,
manufacturing the compression device would be more precise with plastic such
as nylon, delrin etc...)

thanks!
anthony

Orthopaedic Engineerin

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