From: | Kathleen Spencer |
Does anyone know what to do if your floating sections are curling up in tight little cigar rolls in the PBS? I cut 25 micron sections on a cryostat with a steel knife and a roll bar. They are nice and flat when I place them in the wells of PBS , but when I get ready to do IHC a few days later, many of them are curled up so tight they are useless. Any suggestions? Kathleen Spencer