cryosectioning

From:Kathleen Spencer

Does anyone know what to do if your floating sections are curling up in 
tight little cigar rolls in  the PBS? I cut 25 micron sections on a 
cryostat with a steel knife and a roll bar. They are nice and flat when 
I place them in the wells of PBS , but when I get ready to do IHC a few 
days later, many of them are curled up so tight they are useless.

Any suggestions?

Kathleen Spencer





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