Ruthenium Red Protocol

I am trying to identify components of wheat straw stems.  I want to look at
the use of Ruthenium Red and whether this stain will help differentiate
cells and other materials in harvested stems.  I have found a couple
references that provide concentrations of RR to use but I do not have a
complete prep-protocol for use of this stain.  Can anyone provide me with a
staining procedure for ruthenium red and slide prep procedure?


Pete Pryfogle
Idaho National Engr. & Environ. Lab.
Idaho Falls, Idaho  83415-2203
(208) 526-0373

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