Sorry for all the mispelled words.  That's what happens when your doing
your job and answering the histonet.
Rande




rkline@emscience.com on 08/13/2002 11:21:08 AM

To:    "Johnson, Teri" 
cc:    "Histonet (E-mail)" 
Subject:    Re: storage of frozen specimens



Used to store frozen muscles on cork discs for easy mounting on to cryostat
chucks in whirl bags from Fisher.  The bags where great for numbering.  The
Immuno lab would store tissue on chucks wrapped in foil and placed in whirl
bags.  When ;they ran out of chucks they would gently heat the bottom of
the check with warm water and remove the botton.
The cork discs are an easy way to store and easily can be removed from
chucks.  No problem with freezing artifacted either way.  Had freezer boxes
which were number with the accession numbers on the outside in numerical
order for easy location of the specimens.  We used a -70 freezer.

Rande Kline
EM Science




"Johnson, Teri"  on 08/13/2002 10:20:54 AM

To:    "Histonet (E-mail)" 
cc:
Subject:    storage of frozen specimens


Hi again all,

I'm looking for ideas regarding storage of frozen specimens.  I know
everybody does things differently and am interested in what it is you do,
what works well for you.  We store our tissues in a -80 degree C
ultrafreezer.  What I'm currently doing works, but I think I'll tap into
this resource to see if what you are doing is better.

I freeze my tissues in a cryomold.  Sometimes I keep them mounted on the
cryostat chuck if I'm going to be cutting them again in the near future.

Thanks for your help!

Teri Johnson
Managing Director Histology Core Facility
Stowers Institute for Medical Research
1000 E.