Re: ploidy and % S phase

From:Richard Cartun

We do ER (clone 6F11), PR (clone PgR636), Ki-67 (clone MIB-1), and
c-erbB-2 (DAKO pAb).

Richard Cartun

>>> Martha Ward  08/08/02 03:59PM >>>
I was wondering what everyone is doing for their breast cancer
specimens in regards to immunos and/or flow cytometry?  We currently do
ER, PR, Her-2 (Dako), Ki-67, as well as ploidy and %S phase by flow
cytometry.  We have not changed our menu in some time and I wanted to
get a feel for what other institutions are doing.  My impression from
talking with some oncologists is that they do not need the ploidy/% S
phase but we keep getting requests.  I wonder if this is one of those
"we've always done it" situations.  I would appreciate any input while
we evaluate the situation.  Thanks in advance for your input.
Martha Ward, MT (ASCP) QIHC
Wake Forest University Baptist Medical Center
Molecular Diagnostics Lab 

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