Hi Tammy,

That's right, you don't need to use Osmium on these unless you want
the enhanced staining you get with Osmium.  If you have done LM on
sections prior to EM sectioning before, you will see that they stain
darker than tissue that hasn't been processed in Osmium. At least that
is the case with toluidine blue staining.

Karen Pawlowski, Ph.D.
Research Scientist
UT Dallas

Tammy Bailey wrote:
> 
> Hello All,
> If I'm cutting epon sections only to be used for light microscopy (no
> EM)..there is no need during the processing for me to use Osmium...(I fix in
> para/glut)..right??
> 
> tammy
> 
> Tammy Bailey                            / /
> Histology Analysis Lab, VSRC               / /\
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