Hi Noreen,

I've come up with a way to repair these, that I've posted here several times before.  hmmmn, maybe I should patent it :)

This will even work if the tissue has been torn in half - it just takes some strategic positioning.

1.  Cut around the section (through the tape) so that you get a section-shaped piece of tape with the tissue attached to it.
2.  Wet the slide with xylene and position the piece of tape in the correct place on the slide.
3.  Cover this with DPX or other mountant, then a large glass coverslip.  You can still reposition if necessary.
4.  Place it somewhere to dry *thoroughly*, so those Paths can't get their grubby mitts on it and mess up all your hard work!!  *grin*

You can actually do this very quickly once you get the hang of it.  I think my record is about 8 seconds for a slide!

Hope this is of some help to you.  I've had to do this on a few old cases, and while the results aren't perfect if the tissue has torn, it's still much better than finding blocks and recutting, especially with special stains, immuno's etc.

Best of Luck
Aidan


__

aidan schurr  b.m.l.sc
section head, histology
hutt valley district health board
lower hutt
new zealand

aidan.schurr@hvh.co.nz
++64 4 570 9173 (direct)
++64 4 570 9214 (fax)

>>> Noreen Gilman  15/08/2002 >>>
Hi everyone. We have a Sakura tape coverslipper and the only real
problem we have is with some old cases. The tape comes up and brings the
tissue sections with it and we haven't found a way to repair them. Has
anyone solved this problem or do we just have to recut these old cases?
Thanks everyone.
Noreen

Noreen Gilman, BS, HT (ASCP) CLS
Histopathology Supervisor
Broward General Medical Center
Ft. Lauderdale, FL