Re: Making reluctant antibodies work

From:Gayle Callis

Along with Tom's requests, did you 

try different fixatives, different incubation times ie extended even
overnight, deteregents to increase permeability (just discussed along with
fixatives that help permeabilize - acetone 75%/ethyl alcohol 25% on
overnight air dried sections, fix 5 min RT, proceed.  

Are you using sensitive chromogens?? DAB+, with enhancer? NBT- BCIP for alk
phos system.  A kit that increases sensitivity - or picks up weak staining

And to answer another question, yes, we get antibodies made in house that
work with blotting assays, ELISA but not IHC.  


Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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