Go to the BD Pharmingen website and check out their CD marker protocol (for
human, mouse, rat) on frozen sections, it is very basic and works quite
well (we modified it to suit our CD marker staining on murine tissue).  As
for acetone fixation, they only use 10 dips, and some people prefer 10
minutes fixation in cold acetone, 4 C or refrig temperatures after an
overnight air drying of frozen sections.  

SEROTEC and eBiosciences also make CD marker antibodies and have their own
methods for frozen sections, pretty much the same from company to company.
Check out their websites too.

 


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu