Janet

We have been using DAKO's CR3/43 for microglia in human FFPE brain tissue
and a Sigma Lectin, BS-1 (Cat no.L5391) for microglia in rat FFPE brain
tissue. Both require Citrate antigen retrieval.

Cheers

Jim Manavis
Senior Hospital Scientist
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668
FAX: 61-08-8222 3204
e.mail: jim.manavis@imvs.sa.gov.au
Disclaimer: Not this little black duck!

-----Original Message-----
From: Miller, Janet [mailto:jmiller@neurosurgery.wayne.edu]
Sent: Thursday, 8 August 2002 11:55 PM
To: 'histonet@pathology.swmed.edu'
Subject: microglial protocol?


Hello Histo-netters!  I am looking for a microglial protocol for rat and cat
brain tissue.  Does anyone have one that they would be willing to share?
Please let me know!  Thank you in advance!

Janet
jmiller@neurosurgery.wayne.edu