RE: Eosin bleeding out
I have found that if I rinse in a 95% etoh before going into 100% etoh then
the excess eosin is removed but the cytoplasmic counterstain remains. This
has worked will with both Shandon and Richard Allan Eosin.
Just my two cents.
Brad M. Mottishaw HT(ASCP)
University of Utah Medical Center
500 Chipeta way
Salt Lake City, UT 84108
Michael Becker on 08/08/2002 07:50:41 PM
To: HistoNet Server
Subject: Re: Eosin bleeding out
Long time since I posted. Please if anyone out there in HistoLand can tell
me what causes and how can I get rid of Eosin counterstain bleeding out of
frozen tissue sections after they are coverslipped and are drying. Once in
awhile this is a problem. I have not changed any solutions or technique or
mounting media. I use the following protocol:
1. Rapid Fixx-10 to 15 secs
2. Tap water rinse x2
3. Gill 3 Hemotoxylin 35-60 seconds depending on age of stain
4. Tap water rinse at the sink until water is clear
5. Lithium Carbonate Bluing-5 to 7 dips
6. 200 proof Ethanol-10 dips
7. 1% Alcoholic Eosin-2 quick dips
8. 200 proof Ethanol-4 containers-10 dips each
9. 200 proof Ethanol-1 container-1 minute
10.Xylene Substitute-Histosolve for 30 secs to a minute or longer.
Coverslip with Histosolve and a Xylene Substitute mounting media-Histomount
I get all my reagents except Ethanol from Shandon and have been very happy
with staining quality.
Any ideas! Thanks in advance to all who may respond.
Hope summer is treating you all well. Have fun. Be safe.
Sue Becker HTL (ASCP)
c/o Dr. Michael J. Mulvaney
MOHS Micrographic Surgery
Albany, NY 12203
"No one knows noses like MOHS!"
<< Previous Message | Next Message >>