RE: Ag retreival vs. Permeabilizing
I'm with you, that's exactly what I thought as well. But these guys
were working on formalin-fixed, paraffin-embedded tissues!?
So I'm thinking the permeabilizing step is not necessary. I'm going
to try it without any treatment and perhaps with a heat retreival
method and compare results. Thanks for the reply.
Date sent: Tue, 06 Aug 2002 13:41:45 -0400
From: "Morken, Tim"
Subject: RE: Ag retreival vs. Permeabilizing
To: 'Greg Dobbin' , firstname.lastname@example.org
> Detergents such as Triton and saponin "permeabilize" cell membranes by
> making holes in them. This is a physical process that simply makes a "door"
> for the antibody to enter the cell. Usually this is done on whole-cell preps
> such as cell smears and cytospins in which the cell membrane is usually
> intact. It can be done on frozen sections, however it is usually not done on
> paraffin-embedded tissue since that is normally fixed. The process is very
> different than the "antigen retreival" techniques we use on paraffin
> sections which are designed to break formalin/protein bonds.
> Tim Morken
> CDC, Atlanta
> -----Original Message-----
> From: Greg Dobbin [mailto:dobbin@Upei.CA]
> Sent: Tuesday, August 06, 2002 12:55 PM
> To: email@example.com
> Subject: Ag retreival vs. Permeabilizing
> Hello All,
> I found a "Brief Communication" in Vet Pathology (38:116-119;
> 2001) that discusses expression of COX-2 in canine renal
> carcinomas. The authors do not mention antigen retreival, rather,
> they speak of permeabilizing the tissues using triton and
> saponin. Is permeabilizing (as described) a form of antigen
> retreival or does it serve another purpose?
> Has anyone else heard of this before? Explanation??
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave.
> Charlottetown, P.E.I.
> Canada, C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
> "A farmer is a person outstanding in their field."
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Canada, C1A 4P3
"A farmer is a person outstanding in their field."
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